The reading assignments listed below show what you should read before coming to class. You are expected to come to class prepared to discuss the assigned articles.
| SES # | TOPICS | READINGS |
|---|---|---|
| 1 | Introduction | |
| 2 | Blastema formation |
Reading 1Whitehead, G. G., S. Makino, C. L. Lien, and M. T. Keating. "fgf20 is Essential for Initiating Zebrafish Fin Regeneration." Science 310 (2005): 1957-60. Reading 2Yokoyama, H., H. Ogino, C. L. Stoick-Cooper, R. M. Grainger, and R. T. Moon. "Wnt/Beta-Catenin Signaling has an Essential Role in the Initiation of Limb Regeneration." Dev Biol 306 (2007): 170-8. |
| 3 | Progenitor cells I, stem cells |
Reading 1Bagna, J., E. Salo, and C. Auladell. "Regeneration and Pattern Formation in Planarians III. Evidence that Neoblasts are Totipotent Stem Cells and the Source of Blastema Cells." Development 107 (1989): 77-86. Reading 2Reddien, P. W., N. J. Oviedo, J. R. Jennings, J. C. Jenkin, and A. Sánchez Alvarado. "SMEDWI-2 is a PIWI-like Protein that Regulates Planarian Stem Cells." Science 310 (2005): 1327-30. |
| 4 | Progenitor cells II, dedifferentiation |
Reading 1Lo, D. C., F. Allen, and J. P. Brockes. "Reversal of Muscle Differentiation During Urodele Limb Regeneration." PNAS 90 (1993): 7230-4. Reading 2Morrison, J. I., S. Lööf, P. He, and A. Simon. "Salamander Limb Regeneration Involves the Activation of a Multipotent Skeletal Muscle Satellite Cell Population." J Cell Biol 172 (2006): 433-40. |
| 5 | Morphallaxis |
Reading 1Bode, H. R., and S. G. Cumming. "Head Regeneration and Polarity Reversal in Hydra Attenuata can Occur in the Absence of DNA Synthesis." Dev Gen Evol 194 (1984): 79-86. Reading 2González-Estévez, C., D. A. Felix, A. A. Aboobaker, and E. Saló. "Gtdap-1 Promotes Autophagy and is Required for Planarian Remodeling During Regeneration and Starvation." PNAS 104 (2007): 13373-8. |
| 6 | Positional information I, regeneration polarity |
Reading 1Broun, M., L. Gee, B. Reinhardt, and H. R. Bode. "Formation of the Head Organizer in Hydra Involves the Canonical Wnt Pathway." Development 132 (2005): 2907-16. Reading 2Gurley, K. A., J. C. Rink, and A. Sánchez Alvarado. "Beta-Catenin Defines Head versus Tail Identity During Planarian Regeneration and Homeostasis." Science 18 (2008): 323-7. |
| 7 | Positional information II, limb regeneration |
Reading 1Crawford, K., and D. L. Stocum. "Retinoic Acid Coordinately Proximalizes Regenerate Pattern and Blastema Differential Affinity in Axolotl Limbs." Development 102 (1988): 687-98. Reading 2da Silva, S. M., P. B. Gates, and J. P. Brockes. "The Newt Ortholog of CD59 is Implicated in Proximodistal Identity During Amphibian Limb Regeneration." Dev Cell 4 (2002): 547-55. |
| 8 | Positional information III, intercalary regeneration |
Reading 1French, V., P. J. Bryant, and S. V. Bryant. "Pattern Regulation in Epimorphic Fields." Science 193 (1976): 969-81. Reading 2Gibson, M. C., and G. Schubiger. "Hedgehog is Required for Activation of Engrailed During Regeneration of Fragmented Drosophila Imaginal Discs." Development 126 (1999): 1591-9. |
| 9 | Organ regeneration |
Reading 1Lepilina, A., A. N. Coon, K. Kikuchi, J. E. Holdway, R. W. Roberts, C. G. Burns, and K. D. Poss. "A Dynamic Epicardial Injury Response Supports Progenitor Cell Activity During Zebrafish Heart Regeneration." Cell 127 (2006): 607-19. Reading 2Moolten, F. L., and N. L. Bucher. "Regeneration of Rat Liver: Transfer of Humoral Agent by Cross Circulation." Science 158 (1967): 272-4. |
| 10 | Adult stem cells |
Reading 1Gussoni, E., Y. Soneoka, C. D. Strickland, E. A. Buzney, M. K. Khan, A. F. Flint, L. M. Kunkel, and R. C. Mulligan. "Dystrophin Expression in the mdx Mouse Restored by Stem Cell Transplantation." Nature 401 (1999): 390-4. Reading 2Wagers, A. J., R. I. Sherwood, J. L. Christensen, and I. L. Weissman. "Little Evidence for Developmental Plasticity of Adult Hematopoietic Stem Cells." Science 297 (2002): 2256-9. |
| 11 | Homeostasis I, progenitor cells |
Reading 1Dor, Y., J. Brown, O. I. Martinez, and D. A. Melton. "Adult Pancreatic Beta-Cells are Formed by Self-Duplication Rather than Stem-Cell Differentiation." Nature 429 (2004): 41-6. Reading 2Micchelli, C. A., and N. Perrimon. "Evidence that Stem Cells Reside in the Adult Drosophila Midgut Epithelium." Nature 439 (2006): 475-9. |
| 12 | Homeostasis II, stem cell self-renewal and stem cell niche |
Reading 1Molofsky, A. V., R. Pardal, T. Iwashita, I. K. Park, M. F. Clarke, and S. J. Morrison. "Bmi-1 Dependence Distinguishes Neural Stem Cell Self-Renewal from Progenitor Proliferation." Nature 425 (2003): 962-7. Reading 2Tumbar, T., G. Guasch, V. Greco, C. Blanpain, W. E. Lowry, M. Rendl, and E. Fuchs. "Defining the Epithelial Stem Cell Niche in Skin." Science 303 (2004): 359-63. |
| 13 | Regenerative medicine |
Reading 1Takahashi, K., and S. Yamanaka. "Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors." Cell 126 (2006): 663-76. Reading 2Cohn, R. D., et al. "Angiotensin II Type 1 Receptor Blockade Attenuates TGF-Beta—induced Failure of Muscle Regeneration in Multiple Myopathic States." Nat Med 13 (2007): 204-10. |
| 14 | Oral presentations |