Constructing and Screening a Recombinant DNA Library

This session will review how to make a recombinant genomic DNA library and how to use this library to find a specific gene. This session will outline using a library to clone a gene by complementation of a mutant phenotype.

Learning Objectives

• To understand what a recombinant genomic DNA library is and how it is constructed.
• To identify how to use a genomic library to identify a gene of interest.
• To understand how to clone the gene of interest by complementation of a mutant phenoptype.
• To understand the limitations of a genomic library.