- Immune Response–Mechanism
- Antibody/Immunoglobulin Structure
- Antibody Production
- Immune Response–Primary and Secondary
- Immunology–Definitions and Clonal Expansion
- Antibody Variability and VDJ Recombination
- Implications of Antibody Variability
- Humoral and Cellular Immunity
- Macrophage and T Helper Cells I and II
- T Cell–B Cell Interaction
- Neuron Structure
- Neurobiology–Introduction
- Neurobiology–Electrical Signals
- Neurobiology–Concentration Gradients and Membrane Proteins
- Action Potential Mechanism
- Propagating Action Potential
- Synaptic Transmission
- Post-Synaptic Cell / Muscle-Nerve Synapse
- Nerve–Nerve Synapse
- Neural Circuitry
- Viral Infections and Vaccines
- AIDS and HIV
- Autotrophs and Heterotrophs
- Adaptive Immune System
- Humoral Versus Cellular Immunity
- Antibodies and VDJ Recombination
- Clonal Selection, Plasma and Memory Cells
- Pathogen Elimination
- T Cells
- Tolerance of Self Peptides
- Humoral Immunology–Summary
- AIDS and HIV–Summary
- Virus and Immune Response
- Antibodies and Bcells–Practice
- Neuron and Action Potential
- Neurotransmitters
- Neurology–Ions and Channels
- Neurology–Action Potential and Channel Permeability
- Neurology–Resting Potential
- Neurology–Channel States and Mutants
- Neurology–Synapse and Neurotransmitters
- Immunology–MHCI and MHCII
- Immunology–VDJ Recombination
- Immunology–Tcell Selection
- Antibody Structure and Diversity
- Immune Response–Practice I and II
- HIV
- Immunology Review
- Neurobiology–Practice I, II and III
- Antibody Gene and Variability
- Immunization
- Antibody–Antigen Interactions
- Immunology
- Vaccine
- Action Potential
- Immune System–Cells
Immune Response–Mechanism
Antibodies in the blood recognize viral antigens and neutralize the virus.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Antibody/Immunoglobulin Structure
Structure of antibodies-light chain, heavy chain, antigen binding site. Antibodies bind to antigens and neutralize the virus particle.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Antibody Production
Antibodies must be specific to unknown, foreign particles. Each B cells/plasma cell clone produce and secrete a specific type of antibody.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Immune Response–Primary and Secondary
Primary response versus secondary response. Secondary response is much faster and stronger due to memory cells from the primary response. This is the basis for vaccines.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Immunology–Definitions and Clonal Expansion
Definitions of immunoglobulin, antigen, and epitope. External and internal epitopes on antigens can be recognized by antibodies. Recognition of antigen by antibody on the B cell induces the specific B cell to proliferate to produce more specific antibodies.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
- Watch
video clip from Lecture 23 (0:00 - 6:27)
Immunology - Definitions and Clonal Expansion
> Download from iTunes U (MP4 - 131MB)
> Download from Internet Archive (MP4 - 131MB)
Antibody Variability and VDJ Recombination
B cells make different antibodies with the same DNA sequence when various versions of V, D, and J DNA segments combine. Mistakes at the fusion point of the segments also contribute to the variability. Different light chains and heavy chains can combine and the antibody genes are more mutation prone.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Implications of Antibody Variability
Nonsense rearrangements must be eliminated. Antibody cannot react against native proteins (autoimmune disease). The body selectively stimulates B cells that make antibodies that tightly bind to and neutralizes the antigen.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Humoral and Cellular Immunity
Humoral immunity produces soluble antibody molecules and cellular immunity uses cytotoxic T cells to recognize and kill infected cells.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Macrophage and T Helper Cells I and II
Macrophage internalizes foreign particles, presents small peptides on their surfaces, and induce production of specific T helper cells. Use example of bazaar to demonstrate interaction between MHCII of the macrophage and the T cell receptor of the T helper cell.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
T Cell–B Cell Interaction
T helper cells interact with antibodies on the B cell to stimulate it to proliferate. The complex immune system activation involving macrophage, T cell, and B cell reduces the chance of autoimmunity.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Neuron Structure
Different types of receptor neurons. Parts of an individual neuron - cell body, axon, axon hillock, nerve terminals, synapses.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Neurobiology–Introduction
Questions that arise in neurobiology.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Neurobiology–Electrical Signals
Electrical and concentration gradients of ions are responsible for the action potential. Briefly mentions action potential, deplorization and repolarization.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Neurobiology–Concentration Gradients and Membrane Proteins
Concentration gradients of Na+, K+, and Ca++ across the axon membrane are established by ion pumps embedded in the membrane. Energy driven pumps establish an electrical, concentration equilibrium that creates the resting potential at -70mV.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
- Watch
video clip from Lecture 26 (20:07 - 33:25)
Neurobiology - Concentration Gradients and Membrane Proteins
> Download from iTunes U (MP4 - 153MB)
> Download from Internet Archive (MP4 - 153MB)
Action Potential Mechanism
Voltage-gated Na+ and K+ ion channels create the action potential by depolarizing and repolarizing the membrane as ions move across the membrane based on the electrical gradients.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Propagating Action Potential
Action potential is propagated along the axon by locally affecting potential of nearby membrane. Myelin sheathes made by Schwann cells allow faster transmission of the action potential.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Synaptic Transmission
In the pre-synaptic cell, action potential at the nerve terminal induces Ca++ to enter the cell, which helps release of neurotransmitters into the synapse.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Post-Synaptic Cell / Muscle-Nerve Synapse
Neurotransmitters bind to the ligand-gated Na+ ion channels on the post-synaptic cell and induces action potentials. Neurotransmitters are degraded when no longer needed.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Nerve–Nerve Synapse
Nerves can positively or negatively affect the post-synaptic cell. Information from thousands of nerve terminals are integrated at the axon hillock to produce a all-or-nothing action potential response.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Neural Circuitry
Circuitry between sensory neuron, motor neuron, intermediate neurons, and muscle cell. The circuitry allow parts of the body to be coordinated when responding to the same stimulus.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Viral Infections and Vaccines
Life cycle and infection of the polio virus. Jenner and his discovery of vaccine for polio. Vaccines can be made from heat attenuated or formaldehyde treated polio viruses.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
AIDS and HIV
History of AIDS, discovery of HIV, life cycle, infection, and inhibitors of HIV.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Autotrophs and Heterotrophs
Definition, origin, examples, and comparison. Heterotrophs such as humans cannot make certain amino acids and other essential compounds.
7.014 Introductory Biology, Spring 2005
Prof. Penny Chisholm, Prof. Graham Walker, Dr. Julia Khodor, Dr. Michelle Mischke
Course Material Related to This Topic:
Adaptive Immune System
Overview and the four major characteristics of adaptive immunity - diversity, specificity, avoid self-recognition, and memory.
7.014 Introductory Biology, Spring 2005
Prof. Penny Chisholm, Prof. Graham Walker, Dr. Julia Khodor, Dr. Michelle Mischke
Course Material Related to This Topic:
Humoral Versus Cellular Immunity
Comparison of the two types of immunity - characteristics, and cells and molecules involved.
7.014 Introductory Biology, Spring 2005
Prof. Penny Chisholm, Prof. Graham Walker, Dr. Julia Khodor, Dr. Michelle Mischke
Course Material Related to This Topic:
Antibodies and VDJ Recombination
Structure, function, complementarity, and production. 3D molecular image. One plasma cell produces one antibody. Random combinations of various V, D, and J segments of the DNA results in many combinations that encodes different antibody proteins.
7.014 Introductory Biology, Spring 2005
Prof. Penny Chisholm, Prof. Graham Walker, Dr. Julia Khodor, Dr. Michelle Mischke
Course Material Related to This Topic:
Clonal Selection, Plasma and Memory Cells
B cells display their antibody on the surface. Tight binding between the antibody and antigen stimulate the B cell to divide. Division gives rise to plamsa cells that produce antibodies, and memory cells, which are responsible for a faster secondary immune response.
7.014 Introductory Biology, Spring 2005
Prof. Penny Chisholm, Prof. Graham Walker, Dr. Julia Khodor, Dr. Michelle Mischke
Course Material Related to This Topic:
Pathogen Elimination
Mechanisms and cells used to destroy pathogens - bacteria or virus.
7.014 Introductory Biology, Spring 2005
Prof. Penny Chisholm, Prof. Graham Walker, Dr. Julia Khodor, Dr. Michelle Mischke
Course Material Related to This Topic:
T Cells
T cells that recognize foreign peptides displayed by other cells on their major histocompatibility complex (MHC). Cytotoxic T cells kill infected cells; helper T cells activate B cells.
Course Material Related to This Topic:
Tolerance of Self Peptides
Education of immune cells in the thymus. All cells that react to self peptides are killed. Examples of autoimmunity.
7.014 Introductory Biology, Spring 2005
Prof. Penny Chisholm, Prof. Graham Walker, Dr. Julia Khodor, Dr. Michelle Mischke
Course Material Related to This Topic:
Humoral Immunology–Summary
Structure, diversity, and function of antibodies. Production of antibodies through VDJ recombination and the cell types involved. Clonal expansion, and primary and secondary immune responses.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
AIDS and HIV–Summary
Summary of history of AIDS, consequences, and symptoms. Life cycle and infection by HIV and compromised immune system.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
Virus and Immune Response
Immune response to viral infections and how viruses try to evade the system.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
- Complete
practice problem 1 on page 1
- Check solution to
practice problem 1 on page 1
Antibodies and Bcells–Practice
Antibodies as transmembrane receptors that induce B cell proliferation post infection. Memory B cells responsible for rapid second immune response.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
- Complete
practice problems 2, 3 on page 2
- Check solution to
practice problems 2, 3 on page 2
Neuron and Action Potential
Parts and function of the neuron. Ion channels, their state (open, closed, inactivated), and contribution to the action potential.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
- Complete
practice problem 1 on page 1
- Check solution to
practice problem 1 on page 1
Neurotransmitters
Secretion, function, and reduction of neurotransmitters in the synapse.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
- Complete
practice problem 2 on page 4
- Check solution to
practice problem 2 on page 4
Neurology–Ions and Channels
The concentrations of ions and types of channels responsible for the resting potential.
7.013 Introductory Biology, Spring 2006
Prof. Tyler Jacks, Prof. Hazel Sive
Course Material Related to This Topic:
- Complete practice problems 3a–d on page 6
- Check solution to practice problems 3a–d on page 6
Neurology–Action Potential and Channel Permeability
Channels permeability and ion movement during the action potential.
7.013 Introductory Biology, Spring 2006
Prof. Tyler Jacks, Prof. Hazel Sive
Course Material Related to This Topic:
- Complete practice problems 3e–j on page 6
- Check solution to practice problems 3e–j on page 6
Neurology–Resting Potential
Define the resting potential and how it is maintained in a neuron.
7.013 Introductory Biology, Spring 2006
Prof. Tyler Jacks, Prof. Hazel Sive
Course Material Related to This Topic:
- Complete practice problem 3a on page 8
- Check solution to practice problem 3a on page 8
Neurology–Channel States and Mutants
States of various channels during an action potential and the effect of defective mutant channels on the shape of an action potential.
7.013 Introductory Biology, Spring 2006
Prof. Tyler Jacks, Prof. Hazel Sive
Course Material Related to This Topic:
- Complete practice problems 4b–c on page 8
- Check solution to practice problems 4b–c on page 8
Neurology–Synapse and Neurotransmitters
The process of transmitting an action potential from one neuron to the next. Ligands and receptors involved in the process and the affect of receptor up-regulation.
7.013 Introductory Biology, Spring 2006
Prof. Tyler Jacks, Prof. Hazel Sive
Course Material Related to This Topic:
- Complete practice problems 2a–d on page 4
- Check solution to practice problems 2a–d on page 4
Immunology–MHCI and MHCII
Cells that contain MHCI and MHCII and the peptides presented by the MHC molecules.
7.013 Introductory Biology, Spring 2006
Prof. Tyler Jacks, Prof. Hazel Sive
Course Material Related to This Topic:
- Complete
practice problem 3 on page 6
- Check solution to practice problem 3 on page 6
Immunology–VDJ Recombination
Quantitative calculation of the result of VDJ recombination that creates antibodies, and B and T cell receptors.
7.013 Introductory Biology, Spring 2006
Prof. Tyler Jacks, Prof. Hazel Sive
Course Material Related to This Topic:
- Complete
practice problems 4a–f on page 7
- Check solution to practice problems 4a–f on page 7
Immunology–Tcell Selection
Fate of immature and mature T cells that recognize foreign and self antigens.
7.013 Introductory Biology, Spring 2006
Prof. Tyler Jacks, Prof. Hazel Sive
Course Material Related to This Topic:
- Complete
practice problems 4g–i on page 8
- Check solution to practice problems 4g–i on page 8
Antibody Structure and Diversity
Identifying antibody structures and ways to generate diversity.
7.014 Introductory Biology, Spring 2005
Prof. Penny Chisholm, Prof. Graham Walker, Dr. Julia Khodor, Dr. Michelle Mischke
Course Material Related to This Topic:
- Complete practice problem A on page 1
- Check solution to practice problem A on page 1
Immune Response–Practice I and II
Humoral and cellular immune responses involving B cells, T cells, and macrophage. Functions of various molecules. Purpose and function of vaccines.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
- Complete practice problem 2 on page 1
- Check solution to practice problem 2 on page 1
- Complete
practice problems C, D on page 1
- Check solution to practice problems C, D on page 1
HIV
Comprehensive review of the structure, life cycle, and infection of the human immunodeficiency virus.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
- Complete all practice problems on page 1
- Check solution to all practice problems on page 1
Immunology Review
Fill in the blank questions on the basics of immunology - cell types, molecules, humoral response and cellular response.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
- Complete
all practice problems on page 1
- Check solution to all practice problems on page 1
Neurobiology–Practice I, II and III
Action potential, voltage, and various states of ion pumps and channels. Neurotransmitters and chemical synapse.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
- Complete all practice problems on page 1
- Check solution to
all practice problems on page 1
- Complete all practice problems on page 1
- Check solution to all practice problems on page 1
- Complete all practice problems on page 1
- Check solution to all practice problems on page 1
Antibody Gene and Variability
Diagram for different parts of a gene encoding an antibody showing variation produced by VDJ DNA rearrangement and alternative splicing. Antibody structure and memory B cell.
7.014 Introductory Biology, Spring 2005
Prof. Penny Chisholm, Prof. Graham Walker, Dr. Julia Khodor, Dr. Michelle Mischke
Course Material Related to This Topic:
- Complete practice problem A on page 1
- Check solution to practice problem A on page 1
Immunization
Cells and molecules involved in an immune response to viral infection. Pros and cons of vaccinating against chickenpox.
7.014 Introductory Biology, Spring 2005
Prof. Penny Chisholm, Prof. Graham Walker, Dr. Julia Khodor, Dr. Michelle Mischke
Course Material Related to This Topic:
- Complete practice problem B on page 2
- Check solution to practice problem B on page 2
Antibody–Antigen Interactions
Biochemical interactions between antibody and antigen, and DNA mutations that strengthen or weaken these interactions.
Course Material Related to This Topic:
- Complete practice problem C on page 3
- Check solution to practice problem C on page 3
Immunology
Cell types, molecules, and functions of innate, humoral, and cellular immunity.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
- Complete
exam problem 1 on page 2
- Check solution to exam problem 1 on page 2
Vaccine
Production and use of vaccine. Causes of antibody diversity.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
- Complete
exam problem 3 on page 5
Action Potential
Ion channels involved in the action potential and changes in the potential across the axon membrane. Signal transmission at the synapse between two neurons.
7.012 Introduction to Biology, Fall 2004
Prof. Eric Lander, Prof. Robert Weinberg, Dr. Claudette Gardel
Course Material Related to This Topic:
- Complete
exam problem 10 on page 15
Immune System–Cells
True or false questions about functions of cells in the immune system. Production of antibodies against protein from a different species.
7.014 Introductory Biology, Spring 2005
Prof. Penny Chisholm, Prof. Graham Walker, Dr. Julia Khodor, Dr. Michelle Mischke
Course Material Related to This Topic:
- Complete exam problem 7 on page 11

